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英语翻译
A bacterium Pseudomonas sp.KBEL 101 isolated from soil was immobilized within polyacrylamide gel and used for the synthesis of D-p-hydroxyphenylglycine from DL-5-substituted hydantoin.The half-life of immobilized whole cell D-hydantoinase was found to be about 50 hrs.In order to increase the operational stability of immobilized whole cell D-hydantoinase,a carbon or nitrogen source was supplied with the reaction mixture in the continuous stirred tank reactor.As a sole source of carbon,glycerol was found to be most effective,and the activity of immobilized whole cell enzyme was maintained stably during 7 days when 0.1%(W/V) glycerol solution was provided.In the case of nitrogen source,supplying of 0.1%(W/V) yeast extract prolonged the half-life of immobilized whole cell D-hydantoinase to about 25 days.
摘要翻译
A bacterium Pseudomonas sp.KBEL 101 isolated from soil was immobilized within polyacrylamide gel and used for the synthesis of D-p-hydroxyphenylglycine from DL-5-substituted hydantoin.The half-life of immobilized whole cell D-hydantoinase was found to be about 50 hrs.In order to increase the operational stability of immobilized whole cell D-hydantoinase,a carbon or nitrogen source was supplied with the reaction mixture in the continuous stirred tank reactor.As a sole source of carbon,glycerol was found to be most effective,and the activity of immobilized whole cell enzyme was maintained stably during 7 days when 0.1%(W/V) glycerol solution was provided.In the case of nitrogen source,supplying of 0.1%(W/V) yeast extract prolonged the half-life of immobilized whole cell D-hydantoinase to about 25 days.
摘要翻译
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