问题描述：
（生物）详细解释下什么是巢式PCR
现在要做巢式PCR的实验 但是不明白其原理 百度Google上的解释也很简略 还不是特明白 就知道要检测未知的序列 一共要P三次才能最终得到目的片段的序列 谁能给我解释一下巢式PCR的原理或者给篇文献什么的（是巢式PCR不是一般的内种PCR 不要从网上复制下来的答案）
现在要做巢式PCR的实验 但是不明白其原理 百度Google上的解释也很简略 还不是特明白 就知道要检测未知的序列 一共要P三次才能最终得到目的片段的序列 谁能给我解释一下巢式PCR的原理或者给篇文献什么的（是巢式PCR不是一般的内种PCR 不要从网上复制下来的答案）
问题解答：
我来补答
巢式pcr就是通过2次扩增,增加产物特意性.第一次的引物大于产物大小,扩增后,再用第二个引物,目的产物的两端的那个进行扩增,这样子可以提高收率,减少非特异性产物.Nested polymerase chain reaction is a modification of polymerase chain reaction intended to reduce the contamination in products due to the amplification of unexpected primer binding sites.Polymerase chain reaction itself is the process used to amplify DNA samples, via a temperature-mediated DNA polymerase. The products can be used for sequencing or analysis, and this process is a key part of many genetics research laboratories, along with uses in DNA fingerprinting for forensics and other human genetic cases. Conventional PCR requires primers complementary to the termini of the target DNA. A commonly occurring problem is primers binding to incorrect regions of the DNA, giving unexpected products.Nested polymerase chain reaction involves two sets of primers, used in two successive runs of polymerase chain reaction, the second set intended to amplify a secondary target within the first run product.[edit]ProcessesThe target DNA undergoes the first run of polymerase chain reaction with the first set of primers, shown in green. The selection of alternative and similar primer binding sites gives a selection of products, only one containing the intended sequence.The product from the first reaction undergoes a second run with the second set of primers, shown in red. It is very unlikely that any of the unwanted PCR products contain binding sites for both the new primers, ensuring the product from the second PCR has little contamination from unwanted products of primer dimers, hairpins, and alternative primer target sequences.
展开全文阅读