问题描述：
英语翻译
Placement of Cdr2 at the top of the Wee1 regulatory network
suggests that mechanisms regulating Cdr2 could act as inputs to
the mitotic entry control system.We considered that cell polarity
factors positioned at the cell tip might provide spatial cues to limit
Cdr2 distribution to the cell middle.To test this possibility,cell
polarity factor mutants (for3D,mod5D,orb2ts
,orb6ts
,pom1D,tea1D
and tea4D) were screened for defects in Cdr2 localization.This
demonstrated a highly specific role for the protein kinase Pom1,a
conserved member of the dual-specificity tyrosine-phosphorylation
regulated kinase (DYRK) family of kinases
5
.Cdr2 was restricted to
the cellmiddle in wild-type cells,but localized throughout half of the
cell in a pom1D mutant (Fig.3a).pom1D cells grow in a monopolar
manner,and the Cdr2 nodes extended fromthe cellmiddle out to the
non-growing cell end.This strong defect in Cdr2 localization was notobserved in other monopolar or cell polarity mutants
(Supplementary Fig.7),although a more minor defect was found
in tea1D and tea4D mutants,which fail to localize Pom1 to cell
ends
5,18
.Interphase node components downstreamof Cdr2 also loca-
lized to the cell end in pom1D mutants (Supplementary Fig.8; previ-
ously shown for Mid1 (refs 19,20)).
Placement of Cdr2 at the top of the Wee1 regulatory network
suggests that mechanisms regulating Cdr2 could act as inputs to
the mitotic entry control system.We considered that cell polarity
factors positioned at the cell tip might provide spatial cues to limit
Cdr2 distribution to the cell middle.To test this possibility,cell
polarity factor mutants (for3D,mod5D,orb2ts
,orb6ts
,pom1D,tea1D
and tea4D) were screened for defects in Cdr2 localization.This
demonstrated a highly specific role for the protein kinase Pom1,a
conserved member of the dual-specificity tyrosine-phosphorylation
regulated kinase (DYRK) family of kinases
5
.Cdr2 was restricted to
the cellmiddle in wild-type cells,but localized throughout half of the
cell in a pom1D mutant (Fig.3a).pom1D cells grow in a monopolar
manner,and the Cdr2 nodes extended fromthe cellmiddle out to the
non-growing cell end.This strong defect in Cdr2 localization was notobserved in other monopolar or cell polarity mutants
(Supplementary Fig.7),although a more minor defect was found
in tea1D and tea4D mutants,which fail to localize Pom1 to cell
ends
5,18
.Interphase node components downstreamof Cdr2 also loca-
lized to the cell end in pom1D mutants (Supplementary Fig.8; previ-
ously shown for Mid1 (refs 19,20)).
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