A biochemist wants to separate two peptides by ionexchange c

问题描述:

A biochemist wants to separate two peptides by ionexchange chromatography.At the pH of the mobile phase to be used on the column,one peptide (A) has a net charge of \53,due to the presence of more Glu and Asp residues than Arg,Lys,and His residues.Peptide B has a net charge of \31.Which peptide would elute first from a cation-exchange resin?Which would elute first from an anion-exchange resin?
1个回答 分类:综合 2014-09-21

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大概意思是某生物学家想用离子交换色谱分离一个肽,在中性条件下过柱,A的净电荷为3,因为Glu 和 Asp残留物比Arg,Lys,多,b肽净电荷1,问那个先从阳离子交换树脂洗脱下来,那个先从阴离子交换树脂洗脱下来,
 
 
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