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英语翻译
This was performed using the frontal cortex of the first patient (MAL) following the principles expressed by Selkoe et al.(1982).Seventeen grams of the frozen tissue were finely choped and homogenized in 10 vol of 0.1 M TRIS pH 7.5,2% SDS and 0.2% B-ME (beta-mercapto- ethanol).The homogenate was centrifuged at 106,000 g for 120 min at 4 ° C.The resultant pellet was resuspended in the same buffer and centrifuged in the same conditions.The resultant pellet was layered on a gradient composed of 1.2,1.8 and 2 M sucrose layers in 50 mM Tris,1% SDS and centrifuged at 106,000 g for 120 rain at 4 ° C.The material recovered at the 1.8-2 M interface was diluted in 0.1 M TRIS with 2% SDS and pelleted (106,000 g,60 min).The pellet was used for biochemical and immunological investigations.The biochemical analysis revealed that it was an insoluble material of high molecular weight since it was excluded from a 3% acrylamide reticulation SDS gel,according of the method of Laemmli (1970).
This was performed using the frontal cortex of the first patient (MAL) following the principles expressed by Selkoe et al.(1982).Seventeen grams of the frozen tissue were finely choped and homogenized in 10 vol of 0.1 M TRIS pH 7.5,2% SDS and 0.2% B-ME (beta-mercapto- ethanol).The homogenate was centrifuged at 106,000 g for 120 min at 4 ° C.The resultant pellet was resuspended in the same buffer and centrifuged in the same conditions.The resultant pellet was layered on a gradient composed of 1.2,1.8 and 2 M sucrose layers in 50 mM Tris,1% SDS and centrifuged at 106,000 g for 120 rain at 4 ° C.The material recovered at the 1.8-2 M interface was diluted in 0.1 M TRIS with 2% SDS and pelleted (106,000 g,60 min).The pellet was used for biochemical and immunological investigations.The biochemical analysis revealed that it was an insoluble material of high molecular weight since it was excluded from a 3% acrylamide reticulation SDS gel,according of the method of Laemmli (1970).
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