问题描述：
英语翻译
he appropriate working standard solutions for fortification and external calibration curves were prepared daily by diluting the stock solution with HPLC water.The following solvents and reagents were used:(a) sodium dihydrogenphosphate monohydrate (100%; Roth); (b) sodium hydroxide (99%; Merck);
(c) formic acid (98–100%; Merck); (d) sodium hydroxide solution (5 M); (e) sodium hydroxide solution (0.2 M); (f)phosphate buffer (0.05 M,pH 8.5; weigh 6.9 g of sodium dihydrogenphosphate monohydrate,add ca.700 ml of water,adjust pH to 8.5 with 5 M sodium hydroxide solution and dilute to 1000 ml with water); (g) mobile phase A:0.005%(v/v) formic acid in water; (h) mobile phase B:methanol;(i) solid-phase extraction (SPE) cartridges:Oasis HLB(polymeric sorbent) 60 mg (Waters).Silanised pear-shaped flasks:fill flasks with a solution of dimethyldichlorosilane in 100 ml toluene,let stand 30 min,and rinse successively
with toluene,absolute methanol and water.
he appropriate working standard solutions for fortification and external calibration curves were prepared daily by diluting the stock solution with HPLC water.The following solvents and reagents were used:(a) sodium dihydrogenphosphate monohydrate (100%; Roth); (b) sodium hydroxide (99%; Merck);
(c) formic acid (98–100%; Merck); (d) sodium hydroxide solution (5 M); (e) sodium hydroxide solution (0.2 M); (f)phosphate buffer (0.05 M,pH 8.5; weigh 6.9 g of sodium dihydrogenphosphate monohydrate,add ca.700 ml of water,adjust pH to 8.5 with 5 M sodium hydroxide solution and dilute to 1000 ml with water); (g) mobile phase A:0.005%(v/v) formic acid in water; (h) mobile phase B:methanol;(i) solid-phase extraction (SPE) cartridges:Oasis HLB(polymeric sorbent) 60 mg (Waters).Silanised pear-shaped flasks:fill flasks with a solution of dimethyldichlorosilane in 100 ml toluene,let stand 30 min,and rinse successively
with toluene,absolute methanol and water.
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