OT-II:(CD4+---------ovalbumin 323-339 )
These transgenic mice express the mouse alpha-chain and beta-chain T cell receptor that pairs with the CD4 coreceptor and is specific for chicken ovalbumin 323-339 in the context of I-A b.Homozygous mice are viable and fertile.In these mice there is a four-fold increase in the CD4 to CD8 peripheral T cell ratio,and lymph node T cells demonstrate a dose-dependent proliferative response to the specific ovalbumin ligand.These transgenic mice are useful for studying in vivo T cell biology such as TCR-ligand interactions,T cell activation,thymic selection,cross-presentation of antigens,process of thymic selection and central and peripheral T cell tolerance and induction.
OT-I:(CD8+---------ovalbumin 257-264 )
These mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes.The transgenic T cell receptor was designed to recognize ovalbumin residues 257-264 in the context of H2Kb and used to study the role of peptides in positive selection and the response of CD8+ T cells to antigen.Like most TCR transgenics,these mice are somewhat immunodeficient.
