【英语问题】whose shirt is that?可不可以换成whose is that shirt?两者区别在哪里

【英语问题】whose shirt is that?可不可以换成whose is that shirt?两者区别在哪里
如题.讲的清楚一点,
两个句子强调的是哪里
附加一个问题：is it your pen 和 is this your pen有什么区别？区别在哪里？