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英语翻译
In a typical plasmid transformation experiment there will be an extremely heterogeneous mixture of DNA molecules with only a small number consisting of a single vector molecule linked to a single DNA fragment.When E.coli is used for transformation the DNA molecules are mixed with the bacterial cells that have been made competent or permeable by being exposed to a cold solution of calcium chloride (0.1M).The cells are then warmed to 37℃ for 5 minutes to allow the DNA to be taken up.The cells are then transferred to broth and after a short incubation period are able to express the functions of the newly acquired chimeric DNA.The bacteria carrying hybrid molecules are identified by a specific screening procedure and purified:their hybrid plasmids are isolated and characterized,or the new products are isolated and studied.Transformation efficiencies are about 10^6 transformants per μg cloning mixture DNA.
In a typical plasmid transformation experiment there will be an extremely heterogeneous mixture of DNA molecules with only a small number consisting of a single vector molecule linked to a single DNA fragment.When E.coli is used for transformation the DNA molecules are mixed with the bacterial cells that have been made competent or permeable by being exposed to a cold solution of calcium chloride (0.1M).The cells are then warmed to 37℃ for 5 minutes to allow the DNA to be taken up.The cells are then transferred to broth and after a short incubation period are able to express the functions of the newly acquired chimeric DNA.The bacteria carrying hybrid molecules are identified by a specific screening procedure and purified:their hybrid plasmids are isolated and characterized,or the new products are isolated and studied.Transformation efficiencies are about 10^6 transformants per μg cloning mixture DNA.
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