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Cells which can only grow in monolayers are regarded as possessing fewer oncogenes than suspension cells and ,therefore ,such cells are used to produce materials for direct hunman consumption .it has also been shown many times that such cells are capable of producing a wider range of virus types than cells which have been selected to grow in suspension .however ,if a virus or product can be produced from a cell which grows in suspension,then the scale-up of such a process is relatively easy .products for veterinary use are made in this way as well as the α-interferons and the immunobiologicals derived form hybridomas .although the cells used for the latter two products can be shown to be carcinogenic in selected test systems ,they do not pose a sever hazard either in the production phase or when the product has been freed of cellular contaminants prior to use .in table 8.2 there is a summary of the advantage and disadvantage of using monolayer cells .it can be conclude that it is necessary to be in a position to produce commercial quantities of both cell types and that two necessarily different technologies are required .descriptions of these two technologies are presented in the next two sections .
Cells which can only grow in monolayers are regarded as possessing fewer oncogenes than suspension cells and ,therefore ,such cells are used to produce materials for direct hunman consumption .it has also been shown many times that such cells are capable of producing a wider range of virus types than cells which have been selected to grow in suspension .however ,if a virus or product can be produced from a cell which grows in suspension,then the scale-up of such a process is relatively easy .products for veterinary use are made in this way as well as the α-interferons and the immunobiologicals derived form hybridomas .although the cells used for the latter two products can be shown to be carcinogenic in selected test systems ,they do not pose a sever hazard either in the production phase or when the product has been freed of cellular contaminants prior to use .in table 8.2 there is a summary of the advantage and disadvantage of using monolayer cells .it can be conclude that it is necessary to be in a position to produce commercial quantities of both cell types and that two necessarily different technologies are required .descriptions of these two technologies are presented in the next two sections .
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