（1）.选取对数生长期的前列腺癌PC-3系细胞,按照ALDEFLUOR试剂盒说明书的操作方法操作,标记出ALDH1A1高

（1）.选取对数生长期的前列腺癌PC-3系细胞,按照ALDEFLUOR试剂盒说明书的操作方法操作,标记出ALDH1A1高表达的细胞,并确保肿瘤干细胞的活性；
(1) covers logarithm of prostate cancer growth of cells,PC - 3 according to the operation method ALDEFLUOR kit manual operation,marking out the cells ALDH1A1 high expression,and ensure that the activity of tumor stem cells;
将标记好的细胞悬液至于碎冰中,无菌条件下采用流式细胞仪分选出ALDH1A1阳性前列腺癌细胞,确保分选细胞无菌,有活力；
Will mark the good cells levitation liquid as for crushed ice,sterile conditions using flow cytometric analysis ALDH1A1 sorting out,ensure positive prostate cancer cells,energetic sorting cell sterile;
采用DMEM培养基,确保阳性细胞分选出来之后在1小时内接种完毕,以培养形成肿瘤微球体.
Adopt DMEM medium,ensure positive cells after sorting out within an hour,in order to cultivate inoculation finished forming tumors micro spheres.
（2）.确保手术切除人前列腺癌标本热缺血时间不超过45分钟；
(2).Ensure surgical resection specimen who prostate cancer thermal ischemia time no more than 45 minutes;
将人前列腺癌肿块接种到NOD/SCID小鼠的皮下前的整个操作过程一定要在4摄氏度的肾保存液中进行,以确保肿瘤细胞的活性.
Prostate cancer block vaccination to NOD/SCID mice to before the whole operation process of the skin,must be in 4 degrees Celsius in the kidney preservation liquid,to ensure the tumor cell activity.