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No stable metal was added into the water.The background total metal concentrations in the seawater were not determined in this study,but the authors previous study indicated that metal concentration in Hong Kong coastal waters was generally comparable to other estuarine and coastal regions (Wang and Dei,1999).Because radioisotopes (109Cd and 65Zn) were carried in acidic solutions,microliter amounts of 0.1 N Suprapur NaOH were added into the filtered seawater before the addition of radioisotopes to maintain the pH at 8.0.The multi-labeling technique minimized the biological variability associated with different batches of experiments,therefore any variation among metals was purely due to the differences of metal behavior in the cells.
Our preliminary experiments demonstrated that 60% of Zn was precipitated (retained on 0.2 mm membranes) within the first 8 min after the addition of Si at a concentration of 82.9 mM (with a background Si concentration of
16.7 mM),respectively.This was consistent with a previous study (Willey,1977) indicating that Si-Zn coprecipitation occurred at a Si concentration of 71 mM.The proportion of Zn retained on the 0.2 mm filters however decreased exponentially with increasing exposure time.At 22 h,negligible fraction (
至少翻译的通顺点,
No stable metal was added into the water.The background total metal concentrations in the seawater were not determined in this study,but the authors previous study indicated that metal concentration in Hong Kong coastal waters was generally comparable to other estuarine and coastal regions (Wang and Dei,1999).Because radioisotopes (109Cd and 65Zn) were carried in acidic solutions,microliter amounts of 0.1 N Suprapur NaOH were added into the filtered seawater before the addition of radioisotopes to maintain the pH at 8.0.The multi-labeling technique minimized the biological variability associated with different batches of experiments,therefore any variation among metals was purely due to the differences of metal behavior in the cells.
Our preliminary experiments demonstrated that 60% of Zn was precipitated (retained on 0.2 mm membranes) within the first 8 min after the addition of Si at a concentration of 82.9 mM (with a background Si concentration of
16.7 mM),respectively.This was consistent with a previous study (Willey,1977) indicating that Si-Zn coprecipitation occurred at a Si concentration of 71 mM.The proportion of Zn retained on the 0.2 mm filters however decreased exponentially with increasing exposure time.At 22 h,negligible fraction (
至少翻译的通顺点,
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